Enhanced biocompatibility and antibacterial property of polyurethane materials modified with citric acid and chitosan
- Received: 3 Mar 2016
- Accepted: 19 Apr 2016
- Accepted author version posted online: 22 Apr 2016
Citric acid (CA) and chitosan (CS) were covalently immobilized on polyurethane (PU) materials to improve the biocompatibility and antibacterial property. The polyurethane pre-polymer with isocyanate group was synthesized by one pot method, and then grafted with citric acid, followed by blending with polyethersulfone (PES) to prepare the blend membrane through a phase-inversion technique so that chitosan can be grafted from the membrane via esterification and acylation reactions eventually. The native and modified membranes were characterized by attenuated total reflectance-Fourier transform infrared spectroscope (ATR-FTIR), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), water contact angle (WCA) measurement and tensile strength test. Protein adsorption, platelet adhesion, hemolysis assay, activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT) and adsorption of Ca2+ were executed to evaluate the blood compatibility of membranes decorated by CA and CS. Particularly, the antibacterial activities on the modified membrane were evaluated based on a vitro antibacterial test. It could be concluded that the modified membrane had good anticoagulant property and antibacterial property.